Evaluation of sample pooling for screening of SARS CoV-2
To overcome the challenge of nucleic acid (NA) extraction and testing kit availability, an efficient method is urgently needed. The standard reverse transcriptase chain reaction (RT-PCR) test of the SARS CoV-2 was employed targeting the nucleocapsid (N) and open reading frame (ORF1ab) We found that a single positive sample can be amplified and detected in pools of up to 7 samples depending on the cycle threshold.

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