An in-well direct lysis method for rapid detection of SARS-CoV-2 by real time RT-PCR in eSwab specimens
In the SARS-CoV-2 pandemic reagents and utensils for NA purification have been in short supply. This has generated interest in methods that eliminate the need for NA Purification. IGEPAL-CA-630 (IGEPAL) was added to SARS.-Cov-2 MM to 0.3% final concentration and the crude sample was added directly to the. DNA well containing MM. All 30 previously test negative samples remained negative when tested in in-well lysis, direct rRT-PCR.