Improving Detection Efficiency of SARS-CoV-2 Nucleic Acid Testing

SARS-CoV-2 nucleic acid testing (NAT) has been routinely used for diagnosis during this pandemic. There have been concerns about its high false-negative rate. We dissected its detection efficiency with a large COVID-19 cohort study.

Pervasive generation of non-canonical subgenomic RNAs by SARS-CoV-2

SARS-CoV-2 has caused a worldwide pandemic of coronavirus disease 2019 or COVID-19. Coronaviruses generate subgenomic RNAs through a process involving homology between transcriptional regulatory sequences (TRS) Read-mapping was used to determine the 5' and 3' coordinates of all junctions in viral RNAs identified in these samples.

Persistent viral RNA shedding after COVID-19 symptom resolution in older convalescent plasma donors

SARS-CoV-2 is responsible for a worldwide pandemic. Less is known about how long viral shedding occurs once viral symptoms have resolved. We found evidence of persistent viral shedding more than 2 weeks after the resolution of symptoms.

A rapid, cost-effective tailed amplicon method for sequencing SARS-CoV-2

The global COVID-19 pandemic has led to an urgent need for scalable methods for clinical diagnostics and viral tracking. Given the small genome size, the ability to sequence SARS-CoV-2 at scale is limited by the cost and labor associated with making sequencing libraries.

SARS-CoV-2 early infection signature identified potential key infection mechanisms and drug targets

2.3 million people have died worldwide as a result of SARS-CoV-2 infection. The disease mechanism is mostly unknown, and effective treatment options are still out of reach.

Assessing critical gaps in COVID-19 testing capacity: the case of delayed results in Ecuador

WHO's preparedness index omits estimations of actual testing capabilities. Ecuador has one of the highest excess death rates globally. Ecuador had a comparatively low number of confirmed COVID-19 cases.

A prospective, randomized assessment of a spatial orientation device in natural orifice transluminal endoscopic surgery

The Queen's NOTES group has devised a novel method of orientation by using a magnetic device that passes within an endoscope channel allowing for 3-dimensional imaging of the shape and orientation of the endoscope.

An inter-observer agreement study of autofluorescence endoscopy in Barrett's esophagus among expert and non-expert endoscopists

Autofluorescence imaging (AFI) is associated with significant false-positive results during Barrett's surveillance. The aim of this study was to assess the inter-observer agreement (IOA) in identifying AFI-positive lesion

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