Validation of a One-Step Reverse Transcription-Droplet Digital PCR (RT-ddPCR) Approach to Detect and Quantify SARS-CoV-2 RNA in Nasopharyngeal Swabs

Quantitative reverse transcription-PCR (RT-qPCR) is the preferred methodology for viral RNA detection. This study shows that RT-ddPCR allows the detection of as low as 10.3 copies of a SARS-COV-2 E-gene per sample with a higher level of accuracy and precision, especially at low concentration.

Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients

In total, 35/124 (26.6%) samples were RT-PCR positive, with 33/35 positive by NPS. A third or more of pure saliva samples from symptomatic patients were thick, stringy, and difficult to pipet. Our study highlighted the need to optimize collection and processing before saliva can be used for high-volume testing.

Factors associated with viral clearance periods from patients with COVID-19: A retrospective observational cohort study

The study aims to identify the clinical and demographic factors influencing the time taken for viral clearance in patients with COVID-19 to determine the optimal isolation period. Younger patients who recovered from SARS-CoV-2 took less time to clear the virus than older patients.

Transnasal endoscopy is preferred by transoral endoscopy experienced patients

Unsedated transnasal endoscopy (TNE) has been suggested as an alternative to conventional TOE. This study aimed to determine the advantages of TNE in patients who have previously undergone unsedated conventional toE.

Patient-derived measures of GI endoscopy: a meta-narrative review of the literature

The study looked at the literature on methods of assessing patient experience in GI endoscopy. Most measures focused on pain, discomfort, anxiety, and embarrassment. Three studies explored wider aspects of experience, including preparation, unit organization, and endoscopist preference

Safety and efficacy of glucagon as a premedication for upper gastrointestinal endoscopy--a comparative study with butyl scopolamine bromide

Glucagon inhibits digestive motility and is used for endoscopic premedication. Its effect on cardiopulmonary function during endoscopy has not yet been fully investigated. Blood pressure, arterial oxygen saturation, and a number of retching episodes were not different between the groups.

Upper gastrointestinal endoscopy: are preparatory interventions effective?

This study examined the effects of preparatory cognitive and behavioral information on self-confidence, anxiety, and negative affect elicited by an impending upper gastrointestinal endoscopy.

Meta-analysis: erythromycin before endoscopy for acute upper gastrointestinal bleeding

Studies evaluating the effect of erythromycin on patients with acute upper gastrointestinal bleeding (UGIB) had been reported, but the results were inconclusive. Four RCTs including 335 patients were identified and the incidence of the empty stomach was significantly increased in patients receiving the medication

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